How to develop a Standard Operating Procedure for sorting unfixed cells

Standard safety practices for sorting of unfixed cells.

Cell sorting of viable biological specimens has become widespread in laboratories involved in basic and clinical research. As these samples can contain infectious agents, precautions to protect instrument operators and the environment from hazards arising from the use of sorters are paramount. This unit presents a revised and updated version of the biosafety guidelines for sorting of unfixed ce...

Introduction to the biosafety guidelines for sorting of unfixed cells.

Cell sor ting of biologic specimens by flow cytometry has become a widely used technique in r esear ch and clinical laborator ies. Because viable biologic specimens can contain infectious agents, pr ecautions need to be taken to prevent the exposure of operators of flow cytometers to biohazards ar ising from the use of these instruments. The International Society of Analytical Cytology (ISAC) r...

International Society for Analytical Cytology biosafety standard for sorting of unfixed cells.

BACKGROUND Cell sorting of viable biological specimens has become very prevalent in laboratories involved in basic and clinical research. As these samples can contain infectious agents, precautions to protect instrument operators and the environment from hazards arising from the use of sorters are paramount. To this end the International Society of Analytical Cytology (ISAC) took a lead in esta...

How Companies Develop Operating Systems for Innovation

Standard operating procedure for computing pangenome trees

We present the pan-genome tree as a tool for visualizing similarities and differences between closely related microbial genomes within a species or genus. Distance between genomes is computed as a weighted relative Manhattan distance based on gene family presence/absence. The weights can be chosen with emphasis on groups of gene families conserved to various degrees inside the pan-genome. The s...